Increased sucrose levels mediate selective mRNA translation in Arabidopsis.

ABSTRACT:

Background

Protein synthesis is a highly energy demanding process and is regulated according to cellular energy levels. Light and sugar availability affect mRNA translation in plant cells but the specific roles of these factors remain unclear. In this study, sucrose was applied to Arabidopsis seedlings kept in the light or in the dark, in order to distinguish sucrose and light effects on transcription and translation. These were studied using microarray analysis of steady-state mRNA and mRNA bound to translating ribosomes.

Results

Steady-state mRNA levels were affected differently by sucrose in the light and in the dark but general translation increased to a similar extent in both conditions. For a majority of the transcripts changes of the transcript levels were followed by

SUBMITTER: Gamm M 

PROVIDER: S-EPMC4252027 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature