Enhanced lung epithelial specification of human induced pluripotent stem cells on decellularized lung matrix.

ABSTRACT:

Background

Whole-lung scaffolds can be created by perfusion decellularization of cadaveric donor lungs. The resulting matrices can then be recellularized to regenerate functional organs. This study evaluated the capacity of acellular lung scaffolds to support recellularization with lung progenitors derived from human induced pluripotent stem cells (iPSCs).

Methods

Whole rat and human lungs were decellularized by constant-pressure perfusion with 0.1% sodium dodecyl sulfate solution. Resulting lung scaffolds were cryosectioned into slices or left intact. Human iPSCs were differentiated to definitive endoderm, anteriorized to a foregut fate, and then ventralized to a population expressing NK2 homeobox 1 (Nkx2.1). Cells were seeded onto slices and whole lungs, which were maintai

SUBMITTER: Gilpin SE 

PROVIDER: S-EPMC4252658 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature