Optimization of a microfluidic electrophoretic immunoassay using a Peltier cooler.

ABSTRACT: Successful analysis of electrophoretic affinity assays depends strongly on the preservation of the affinity complex during separations. Elevated separation temperatures due to Joule heating promotes complex dissociation leading to a reduction in sensitivity. Affinity assays performed in glass microfluidic devices may be especially prone to this problem due to poor heat dissipation due to the low thermal conductivity of glass and the large amount of bulk material surrounding separation channels. To address this limitation, a method to cool a glass microfluidic chip for performing an affinity assay for insulin was achieved by a Peltier cooler localized over the separation channel. The Peltier cooler allowed for rapid stabilization of temperatures, with 21°C the lowest temperature that was po

SUBMITTER: Mukhitov N 

PROVIDER: S-EPMC4252761 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature