Cellular mechanism of premature ventricular contraction-induced cardiomyopathy.

ABSTRACT:

Background

Frequent premature ventricular contractions (PVCs) are associated with increased risk of sudden cardiac death and can cause secondary cardiomyopathy.

Objective

We sought to determine the mechanism(s) responsible for prolonged refractory period and left ventricular (LV) dysfunction demonstrated in our canine model of PVC-induced cardiomyopathy.

Methods

Single myocytes were isolated from LV free wall of PVC and control canines and used for patch-clamp recording, intracellular Ca(2+) measurements, and immunocytochemistry/confocal microscopy. LV tissues adjacent to the area of myocyte isolation were used for the immunoblot quantification of protein expression.

Results

In the PVC group, LV ejection fraction decreased from 57.6% ± 1.5% to 30.4% ± 3.1% afte

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4252777 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature