Novel robust biomarkers for human bladder cancer based on activation of intracellular signaling pathways.

ABSTRACT: We recently proposed a new bioinformatic algorithm called OncoFinder for quantifying the activation of intracellular signaling pathways. It was proved advantageous for minimizing errors of high-throughput gene expression analyses and showed strong potential for identifying new biomarkers. Here, for the first time, we applied OncoFinder for normal and cancerous tissues of the human bladder to identify biomarkers of bladder cancer. Using Illumina HT12v4 microarrays, we profiled gene expression in 17 cancer and seven non-cancerous bladder tissue samples. These experiments were done in two independent laboratories located in Russia and Canada. We calculated pathway activation strength values for the investigated transcriptomes and identified signaling pathways that were regulated differently i

SUBMITTER: Lezhnina K 

PROVIDER: S-EPMC4253415 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature