The use of three-dimensional printing to produce in vitro slice chambers.

ABSTRACT:

Background

In recent years, 3D printing technology has become inexpensive and simple enough that any lab can own and use one of these printers.

New method

We explored the potential use of 3D printers for quickly and easily producing in vitro slice chambers for patch clamp electrophysiology. Slice chambers were produced using five available plastics: ABS, PLA, Nylon 618, Nylon 680, and T-glase. These "lab-made" chambers were also made using stereolithography through a professional printing service (Shapeways). This study measured intrinsic membrane properties of neurons in the brain stem pedunculopontine nucleus (PPN) and layer V pyramidal neurons in retrosplenial cortex.

Results

Nylon 680 and T-glase significantly hyperpolarized PPN neurons. ABS increased input resist

SUBMITTER: Hyde J 

PROVIDER: S-EPMC4253646 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature