Calcium-activated chloride channels anoctamin 1 and 2 promote murine uterine smooth muscle contractility.

ABSTRACT:

Objective

To determine the presence of calcium activated chloride channels anoctamin 1 (ANO1) and 2 (ANO2) in human and murine uterine smooth muscle (MUSM) and evaluate the physiologic role for these ion channels in murine myometrial contractility.

Study design

We performed reverse transcription polymerase chain reaction to determine whether ANO1 and 2 are expressed in human and murine uterine tissue to validate the study of this protein in mouse models. Immunohistochemical staining of ANO1 and 2 was then performed to determine protein expression in murine myometrial tissue. The function of ANO1 and 2 in murine uterine tissue was evaluated using electrophysiologic studies, organ bath, and calcium flux experiments.

Results

ANO1 and 2 are expressed in human and MUSM cel

SUBMITTER: Bernstein K 

PROVIDER: S-EPMC4253652 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature