Proteomic analysis of the mitochondria from embryonic and postnatal rat brains reveals response to developmental changes in energy demands.

ABSTRACT: Many biological processes converge on the mitochondria. In such systems, where many pathways converge, manipulation of the components can produce varied and far-reaching effects. Due to the centrality of the mitochondria in many cellular pathways, we decided to investigate the brain mitochondrial proteome during early development. Using a SWATH mass spectrometry-based technique, we were able to identify vast proteomic alterations between whole brain mitochondria from rats at embryonic day 18 compared to postnatal day 7. These findings include statistically significant alterations in proteins involved in glycolysis and mitochondrial trafficking/dynamics. Additionally, bioinformatic analysis enabled the identification of HIF1A and XBP1 as upstream transcriptional regulators of many of the di

SUBMITTER: Villeneuve LM 

PROVIDER: S-EPMC4253732 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature