Association between specific timothy grass antigens and changes in TH1- and TH2-cell responses following specific immunotherapy.

ABSTRACT:

Background

Different populations of T cells are involved in the pathogenesis of allergic diseases.

Objective

We investigated changes in TH-cell populations in patients with allergies after specific immunotherapy (SIT).

Methods

PBMCs were isolated from patients with allergies who received SIT and those who did not (controls). We tested the ability of peptides from 93 timothy grass (TG) proteins to induce T-cell responses (cytokine production). We used ELISPOT and staining assays for intracellular cytokines to measure the production of IL-4, IL-5, IL-13, IFN-γ, and IL-10.

Results

Compared with PBMCs from controls, PBMCs from patients who received SIT produced lower levels of TH2 cytokines on incubation with several different TG peptides. These data were used to s

SUBMITTER: Schulten V 

PROVIDER: S-EPMC4253970 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature