Genome-wide analysis of miRNA and mRNA transcriptomes during amelogenesis.

ABSTRACT:

Background

In the rodent incisor during amelogenesis, as ameloblast cells transition from secretory stage to maturation stage, their morphology and transcriptome profiles change dramatically. Prior whole genome transcriptome analysis has given a broad picture of the molecular activities dominating both stages of amelogenesis, but this type of analysis has not included miRNA transcript profiling. In this study, we set out to document which miRNAs and corresponding target genes change significantly as ameloblasts transition from secretory- to maturation-stage amelogenesis.

Results

Total RNA samples from both secretory- and maturation-stage rat enamel organs were subjected to genome-wide miRNA and mRNA transcript profiling. We identified 59 miRNAs that were differentially expre

SUBMITTER: Yin K 

PROVIDER: S-EPMC4254193 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature