Redox-based probes as tools to monitor oxidized protein tyrosine phosphatases in living cells.

ABSTRACT: Reversible oxidation of protein tyrosine phosphatases (PTPs) has emerged as an important regulatory mechanism whereby reactive oxygen species (ROS) inactivates the PTP and promotes phosphorylation and induction of the signaling cascade. The lack of sensitive and robust methods to directly detect oxidized PTPs has made it difficult to understand the effects that PTP oxidative inactivation play in redox signaling. We report the use of redox-based probes to directly detect oxidized PTPs in a cellular context, which highlights the importance of direct approaches to assist in the study of physiological and pathophysiological PTP activity in redox regulation. We also demonstrate, as a proof-of-concept, that these redox-based probes serve as prototypes for the design and development of a new clas

SUBMITTER: Garcia FJ 

PROVIDER: S-EPMC4254195 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature