Coordinated messenger RNA/microRNA changes in fibroblasts of patients with major depression.

ABSTRACT:

Background

Peripheral biomarkers for major psychiatric disorders have been an elusive target for the last half a century. Dermal fibroblasts are a simple, relevant, and much underutilized model for studying molecular processes of patients with affective disorders, as they share considerable similarity of signal transduction with neuronal tissue.

Methods

Cultured dermal fibroblast samples from patients with major depressive disorder (MDD) and matched control subjects (n = 16 pairs, 32 samples) were assayed for genome-wide messenger RNA (mRNA) expression using microarrays. In addition, a simultaneous quantitative polymerase chain reaction-based assessment of >1000 microRNA (miRNA) species was performed. Finally, to test the relationship between the mRNA-miRNA expression change

SUBMITTER: Garbett KA 

PROVIDER: S-EPMC4254393 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature