Development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus.

ABSTRACT:

Background

Respiratory Syncytial Virus (RSV) is an important human respiratory pathogen, particularly of infants and older adults, and despite several decades of research and development, no licensed vaccine is available. Studies have confirmed that enhancement of RSV disease does not occur after inoculation with RSV live-attenuated vaccine candidates, making such vaccines preferable. In this paper, reverse genetics was used to construct two recombinant viruses, a recombinant Long strain (rLong) and rLong-∆G-EGFP; rLong-∆G-EGFP is a recombinant mutant in which G was replaced with the EGFP gene, based on the Long strain of RSV.

Results

Both rLong and rLong-∆G-EGFP were constructed successfully and recovered in Hep-2 cells, and autofluorescence was observed in rLong-∆G-EGFP-in

SUBMITTER: Hu B 

PROVIDER: S-EPMC4254404 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature