A comprehensive biophysical description of pairwise epistasis throughout an entire protein domain.

ABSTRACT:

Background

Nonadditivity in fitness effects from two or more mutations, termed epistasis, can result in compensation of deleterious mutations or negation of beneficial mutations. Recent evidence shows the importance of epistasis in individual evolutionary pathways. However, an unresolved question in molecular evolution is how often and how significantly fitness effects change in alternative genetic backgrounds.

Results

To answer this question, we quantified the effects of all single mutations and double mutations between all positions in the IgG-binding domain of protein G (GB1). By observing the first two steps of all possible evolutionary pathways using this fitness profile, we were able to characterize the extent and magnitude of pairwise epistasis throughout an entire pr

SUBMITTER: Olson CA 

PROVIDER: S-EPMC4254498 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature