Combining an optical resonance biosensor with enzyme activity kinetics to understand protein adsorption and denaturation.

ABSTRACT: Understanding protein adsorption and resultant conformation changes on modified and unmodified silicon dioxide surfaces is a subject of keen interest in biosensors, microfluidic systems and for medical diagnostics. However, it has been proven difficult to investigate the kinetics of the adsorption process on these surfaces as well as understand the topic of the denaturation of proteins and its effect on enzyme activity. A highly sensitive optical whispering gallery mode (WGM) resonator was used to study a catalytic enzyme's adsorption processes on different silane modified glass substrates (plain glass control, DETA, 13 F, and SiPEG). The WGM sensor was able to obtain high resolution kinetic data of glucose oxidase (GO) adsorption with sensitivity of adsorption better than that possible wi

SUBMITTER: Wilson KA 

PROVIDER: S-EPMC4254915 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature