Differentially charged isoforms of apolipoprotein E from human blood are potential biomarkers of Alzheimer's disease.

ABSTRACT:

Introduction

Alzheimer's disease (AD) is the major cause of dementia among the elderly. Finding blood-based biomarkers for disease diagnosis and prognosis is urgently needed.

Methods

We studied protein distributions in brain tissues, cerebrospinal fluid (CSF), and blood of AD patients by using proteomics and a new proteomic method that we call "2D multiplexed Western blot" (2D mxWd). This method allows us to determine in multiple samples the electrophoretic patterns of protein isoforms with different isoelectric points.

Results

Apolipoprotein E (ApoE) displays a unique distribution of electrophoretic isoforms in the presence of AD and also a unique pattern specific to the APOE genotype.

Conclusions

The isoelectric distribution of differentially charged ApoE iso

SUBMITTER: Alzate O 

PROVIDER: S-EPMC4255367 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature