Association of Streptomyces community composition determined by PCR-denaturing gradient gel electrophoresis with indoor mold status.

ABSTRACT: Both Streptomyces species and mold species have previously been isolated from moisture-damaged building materials; however, an association between these two groups of microorganisms in indoor environments is not clear. In this study, we used a culture-independent method, PCR-denaturing gradient gel electrophoresis (PCR-DGGE), to investigate the composition of the Streptomyces community in house dust. Twenty-three dust samples each from two sets of homes categorized as high-mold and low-mold based on mold-specific quantitative PCR analysis were used in the study. Taxonomic identification of prominent bands was performed by cloning and sequencing. Associations between DGGE amplicon band intensities and home mold status were assessed using univariate analyses as well as multivariate recursive

SUBMITTER: Johansson E 

PROVIDER: S-EPMC4255558 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature