Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics.

ABSTRACT: The fatal neurodegenerative disorders amyotrophic lateral sclerosis and spinal muscular atrophy are, respectively, the most common motoneuron disease and genetic cause of infant death. Various in vitro model systems have been established to investigate motoneuron disease mechanisms, in particular immortalized cell lines and primary neurons. Using quantitative mass-spectrometry-based proteomics, we compared the proteomes of primary motoneurons to motoneuron-like cell lines NSC-34 and N2a, as well as to non-neuronal control cells, at a depth of 10,000 proteins. We used this resource to evaluate the suitability of murine in vitro model systems for cell biological and biochemical analysis of motoneuron disease mechanisms. Individual protein and pathway analysis indicated substantial difference

SUBMITTER: Hornburg D 

PROVIDER: S-EPMC4256493 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature