Proteogenomics of Gammarus fossarum to document the reproductive system of amphipods.

ABSTRACT: Because of their ecological importance, amphipod crustacea are employed worldwide as test species in environmental risk assessment. Although proteomics allows new insights into the molecular mechanisms related to the stress response, such investigations are rare for these organisms because of the lack of comprehensive protein sequence databases. Here, we propose a proteogenomic approach for identifying specific proteins of the freshwater amphipod Gammarus fossarum, a keystone species in European freshwater ecosystems. After deep RNA sequencing, we created a comprehensive ORF database. We identified and annotated the most relevant proteins detected through a shotgun tandem mass spectrometry analysis carried out on the proteomes from three major tissues involved in the organism's reproductiv

SUBMITTER: Trapp J 

PROVIDER: S-EPMC4256509 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature