Huntingtin is required for ER-to-Golgi transport and for secretory vesicle fusion at the plasma membrane.

ABSTRACT: Huntingtin is a large membrane-associated scaffolding protein that associates with endocytic and exocytic vesicles and modulates their trafficking along cytoskeletal tracks. Although the progression of Huntington's disease is linked to toxic accumulation of mutant huntingtin protein, loss of wild-type huntingtin function might also contribute to neuronal cell death, but its precise function is not well understood. Therefore, we investigated the molecular role of huntingtin in exocytosis and observed that huntingtin knockdown in HeLa cells causes a delay in endoplasmic reticulum (ER)-to-Golgi transport and a reduction in the number of cargo vesicles leaving the trans-Golgi network. In addition, we found that huntingtin is required for secretory vesicle fusion at the plasma membrane. Similar

SUBMITTER: Brandstaetter H 

PROVIDER: S-EPMC4257002 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature