An in vitro model of human neocortical development using pluripotent stem cells: cocaine-induced cytoarchitectural alterations.

ABSTRACT: Neocortical development involves ordered specification of forebrain cortical progenitors to various neuronal subtypes, ultimately forming the layered cortical structure. Modeling of this process using human pluripotent stem cells (hPSCs) would enable mechanistic studies of human neocortical development, while providing new avenues for exploration of developmental neocortical abnormalities. Here, we show that preserving hPSCs aggregates - allowing embryoid body formation - while adding basic fibroblast growth factor (bFGF) during neuroepithelial development generates neural rosettes showing dorsal forebrain identity, including Mash1(+) dorsal telencephalic GABAergic progenitors. Structures that mirrored the organization of the cerebral cortex formed after rosettes were seeded and cultured f

SUBMITTER: Kindberg AA 

PROVIDER: S-EPMC4257008 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature