Expanding the potential of standard flow cytometry by extracting fluorescence lifetimes from cytometric pulse shifts.

ABSTRACT: Fluorescence lifetime measurements provide information about the fluorescence relaxation, or intensity decay, of organic fluorophores, fluorescent proteins, and other inorganic molecules that fluoresce. The fluorescence lifetime is emerging in flow cytometry and is helpful in a variety of multiparametric, single cell measurements because it is not impacted by nonlinearity that can occur with fluorescence intensity measurements. Yet time-resolved cytometry systems rely on major hardware modifications making the methodology difficult to reproduce. The motivation of this work is, by taking advantage of the dynamic nature of flow cytometry sample detection and applying digital signal processing methods, to measure fluorescence lifetimes using an unmodified flow cytometer. We collect a new life

SUBMITTER: Cao R 

PROVIDER: S-EPMC4257068 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature