Regulatory mechanism of endothelin receptor B in the cerebral arteries after focal cerebral ischemia.

ABSTRACT:

Background and purpose

Increased expression of endothelin receptor type B (ETBR), a vasoactive receptor, has recently been implied in the reduced cerebral blood flow and exacerbated neuronal damage after ischemia-reperfusion (I/R). The study explores the regulatory mechanisms of ETBR to identify drug targets to restore normal cerebral artery contractile function as part of successful neuroprotective therapy.

Methods

We have employed in vitro methods on human and rat cerebral arteries to study the regulatory mechanisms and the efficacy of target selective inhibitor, Mithramycin A (MitA), to block the ETBR mediated contractile properties. Later, middle cerebral artery occluded (MCAO) rats were used to substantiate the observations. Quantative PCR, immunohistochemistry, western

SUBMITTER: Grell AS 

PROVIDER: S-EPMC4257531 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature