Correlating intravital multi-photon microscopy to 3D electron microscopy of invading tumor cells using anatomical reference points.

ABSTRACT: Correlative microscopy combines the advantages of both light and electron microscopy to enable imaging of rare and transient events at high resolution. Performing correlative microscopy in complex and bulky samples such as an entire living organism is a time-consuming and error-prone task. Here, we investigate correlative methods that rely on the use of artificial and endogenous structural features of the sample as reference points for correlating intravital fluorescence microscopy and electron microscopy. To investigate tumor cell behavior in vivo with ultrastructural accuracy, a reliable approach is needed to retrieve single tumor cells imaged deep within the tissue. For this purpose, fluorescently labeled tumor cells were subcutaneously injected into a mouse ear and imaged using two-pho

SUBMITTER: Karreman MA 

PROVIDER: S-EPMC4257674 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature