Identification of host proteins interacting with the integrin-like A domain of Toxoplasma gondii micronemal protein MIC2 by yeast-two-hybrid screening.

ABSTRACT:

Background

Toxoplasma gondii is an obligate intracellular protozoan, causing the important zoonosis toxoplasmosis. This parasite utilizes a unique form of locomotion called gliding motility to find and invade host cells. The micronemal adhesin MIC2 plays critical roles in these processes by binding to substrates and host cell receptors using its extracellular adhesive domains. Although MIC2 is known to mediate important interactions between parasites and host cells during invasion, the specific host proteins interacting with MIC2 have not been clearly identified. In this study, we used a yeast-two-hybrid system to search for host proteins that interact with MIC2.

Methods

Different adhesive domains of MIC2 were cloned into the pGBKT7 vector and expressed in fusion with the GA

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4258286 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature