Transcriptome analysis of the human corneal endothelium.

ABSTRACT:

Purpose

To comprehensively characterize human corneal endothelial cell (HCEnC) gene expression and age-dependent differential gene expression and to identify expressed genes mapped to chromosomal loci associated with the corneal endothelial dystrophies posterior polymorphous corneal dystrophy (PPCD)1, Fuchs endothelial corneal dystrophy (FECD)4, and X-linked endothelial dystrophy (XECD).

Methods

Total RNA was isolated from ex vivo corneal endothelium obtained from six pediatric and five adult donor corneas. Complementary DNA was hybridized to the Affymetrix GeneChip 1.1ST array. Data analysis was performed using Partek Genomics Suite software, and differentially expressed genes were validated by digital molecular barcoding technology.

Results

Transcripts corresponding

SUBMITTER: Frausto RF 

PROVIDER: S-EPMC4258927 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature