Initiation of DNA replication from non-canonical sites on an origin-depleted chromosome.

ABSTRACT: Eukaryotic DNA replication initiates from multiple sites on each chromosome called replication origins (origins). In the budding yeast Saccharomyces cerevisiae, origins are defined at discrete sites. Regular spacing and diverse firing characteristics of origins are thought to be required for efficient completion of replication, especially in the presence of replication stress. However, a S. cerevisiae chromosome III harboring multiple origin deletions has been reported to replicate relatively normally, and yet how an origin-deficient chromosome could accomplish successful replication remains unknown. To address this issue, we deleted seven well-characterized origins from chromosome VI, and found that these deletions do not cause gross growth defects even in the presence of replication inhi

SUBMITTER: Bogenschutz NL 

PROVIDER: S-EPMC4259332 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature