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Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae).


ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Chloroplast microsatellite markers were developed for Festuca rubra to examine its population genetic characteristics, taxonomy, and coevolution with its endophyte Epichloë festucae. • METHODS AND RESULTS:Thirteen polymorphic markers were identified from the chloroplast genome of a F. ovina accession and intergenic chloroplast sequences of F. rubra accessions. They amplified a total of 65 alleles in a sample of 93 individuals of F. rubra originating from six different populations located in the Faroe Islands, Finland, Greenland, Norway, and Spain. • CONCLUSIONS:The developed microsatellite primer pairs can be used by researchers in population genetic and taxonomic studies, and by plant breeders in breeding programs on grasses.

SUBMITTER: von Crautlein M 

PROVIDER: S-EPMC4259459 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae).

von Cräutlein Maria M   Korpelainen Helena H   Helander Marjo M   Väre Henry H   Saikkonen Kari K  

Applications in plant sciences 20141204 12


<h4>Unlabelled</h4><h4>Premise of the study</h4>Chloroplast microsatellite markers were developed for Festuca rubra to examine its population genetic characteristics, taxonomy, and coevolution with its endophyte Epichloë festucae. •<h4>Methods and results</h4>Thirteen polymorphic markers were identified from the chloroplast genome of a F. ovina accession and intergenic chloroplast sequences of F. rubra accessions. They amplified a total of 65 alleles in a sample of 93 individuals of F. rubra ori  ...[more]

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