The transcriptome of Legionella pneumophila-infected human monocyte-derived macrophages.

ABSTRACT:

Background

Legionella pneumophila is an intracellular bacterial pathogen that invades and replicates within alveolar macrophages through injection of ∼ 300 effector proteins by its Dot/Icm type IV translocation apparatus. The bona fide F-box protein, AnkB, is a nutritional virulence effector that triggers macrophages to generate a surplus of amino acids, which is essential for intravacuolar proliferation. Therefore, the ankB mutant represents a novel genetic tool to determine the transcriptional response of human monocyte-derived macrophages (hMDMs) to actively replicating L. pneumophila.

Methodology/principal findings

Here, we utilized total human gene microarrays to determine the global transcriptional response of hMDMs to infection by wild type or the ankB mutant of L. pn

SUBMITTER: Price CT 

PROVIDER: S-EPMC4259488 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature