Carboxy terminus of GATA4 transcription factor is required for its cardiogenic activity and interaction with CDK4.

ABSTRACT: GATA4-6 transcription factors regulate numerous aspects of development and homeostasis in multiple tissues of mesodermal and endodermal origin. In the heart, the best studied of these factors, GATA4, has multiple distinct roles in cardiac specification, differentiation, morphogenesis, hypertrophy and survival. To improve understanding of how GATA4 achieves its numerous roles in the heart, here we have focused on the carboxy-terminal domain and the residues required for interaction with cofactors FOG2 and Tbx5. We present evidence that the carboxy terminal region composed of amino acids 362-400 is essential for mediating cardiogenesis in Xenopus pluripotent explants and embryos. In contrast, the same region is not required for endoderm-inducing activity of GATA4. Further evidence is present

SUBMITTER: Gallagher JM 

PROVIDER: S-EPMC4259525 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature