Ontology highlight
ABSTRACT:
SUBMITTER: Reuter M
PROVIDER: S-EPMC4259808 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Reuter Marcel M Zelensky Alex A Smal Ihor I Meijering Erik E van Cappellen Wiggert A WA de Gruiter H Martijn HM van Belle Gijsbert J GJ van Royen Martin E ME Houtsmuller Adriaan B AB Essers Jeroen J Kanaar Roland R Wyman Claire C
The Journal of cell biology 20141201 5
Genome maintenance by homologous recombination depends on coordinating many proteins in time and space to assemble at DNA break sites. To understand this process, we followed the mobility of BRCA2, a critical recombination mediator, in live cells at the single-molecule level using both single-particle tracking and fluorescence correlation spectroscopy. BRCA2-GFP and -YFP were compared to distinguish diffusion from fluorophore behavior. Diffusive behavior of fluorescent RAD51 and RAD54 was determ ...[more]