Retinal vasculature of adult zebrafish: in vivo imaging using confocal scanning laser ophthalmoscopy.

ABSTRACT: Over the past 3 decades the zebrafish (Danio rerio) has become an important biomedical research species. As their use continues to grow additional techniques and tools will be required to keep pace with ongoing research using this species. In this paper we describe a novel method for in vivo imaging of the retinal vasculature in adult animals using a commercially available confocal scanning laser ophthalmoscope (SLO). With this instrumentation, we demonstrate the ability to distinguish diverse vascular phenotypes in different transgenic GFP lines. In addition this technology allows repeated visualization of the vasculature in individual zebrafish over time to document vascular leakage progression and recovery induced by intraocular delivery of proteins that induce vascular permeability. SL

SUBMITTER: Bell BA 

PROVIDER: S-EPMC4259836 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature