Relating influenza virus membrane fusion kinetics to stoichiometry of neutralizing antibodies at the single-particle level.

ABSTRACT: The ability of antibodies binding the influenza hemagglutinin (HA) protein to neutralize viral infectivity is of key importance in the design of next-generation vaccines and for prophylactic and therapeutic use. The two antibodies CR6261 and CR8020 have recently been shown to efficiently neutralize influenza A infection by binding to and inhibiting the influenza A HA protein that is responsible for membrane fusion in the early steps of viral infection. Here, we use single-particle fluorescence microscopy to correlate the number of antibodies or antibody fragments (Fab) bound to an individual virion with the capacity of the same virus particle to undergo membrane fusion. To this end, individual, infectious virus particles bound by fluorescently labeled antibodies/Fab are visualized as they

SUBMITTER: Otterstrom JJ 

PROVIDER: S-EPMC4260548 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature