Inhibition of tumor cell proliferation and motility by fibroblasts is both contact and soluble factor dependent.

ABSTRACT: Normal human and murine fibroblasts can inhibit proliferation of tumor cells when cocultured in vitro. The inhibitory capacity varies depending on the donor and the site of origin of the fibroblast. We showed previously that effective inhibition requires formation of a morphologically intact fibroblast monolayer before seeding of the tumor cells. Here we show that inhibition is extended to motility of tumor cells and we dissect the factors responsible for these inhibitory functions. We find that inhibition is due to two different sets of molecules: (i) the extracellular matrix (ECM) and other surface proteins of the fibroblasts, which are responsible for contact-dependent inhibition of tumor cell proliferation; and (ii) soluble factors secreted by fibroblasts when confronted with tumor cel

SUBMITTER: Alkasalias T 

PROVIDER: S-EPMC4260581 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature