Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells.

ABSTRACT: F1Fo ATP synthase is present in all organisms and is predominantly located on the inner membrane of mitochondria in eukaryotic cells. The present study demonstrated that ATP synthase and electron transport chain complexes were ectopically expressed on the surface of breast cancer cells and could serve as a potent anticancer target. We investigated the anticancer effects of the ATP synthase inhibitor citreoviridin on breast cancer cells through proteomic approaches and revealed that differentially expressed proteins in cell cycle regulation and in the unfolded protein response were functionally enriched. We showed that citreoviridin triggered PERK-mediated eIF2α phosphorylation, which in turn attenuated general protein synthesis and led to cell cycle arrest in the G0/G1 phase. We further sh

SUBMITTER: Chang HY 

PROVIDER: S-EPMC4260757 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature