Vector modifications to eliminate transposase expression following piggyBac-mediated transgenesis.

ABSTRACT: Transgene insertion plays an important role in gene therapy and in biological studies. Transposon-based systems that integrate transgenes by transposase-catalyzed "cut-and-paste" mechanism have emerged as an attractive system for transgenesis. Hyperactive piggyBac transposon is particularly promising due to its ability to integrate large transgenes with high efficiency. However, prolonged expression of transposase can become a potential source of genotoxic effects due to uncontrolled transposition of the integrated transgene from one chromosomal locus to another. In this study we propose a vector design to decrease post-transposition expression of transposase and to eliminate the cells that have residual transposase expression. We design a single plasmid construct that combines the transpo

SUBMITTER: Chakraborty S 

PROVIDER: S-EPMC4261183 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature