Enrichment of calcifying extracellular vesicles using density-based ultracentrifugation protocol.

ABSTRACT: Calcifying extracellular vesicles (EVs) released from cells within atherosclerotic plaques have received increased attention for their role in mediating vascular calcification, a major predictor of cardiovascular morbidity and mortality. However, little is known about the difference between this pathologic vesicle population and other EVs that contribute to physiological cellular processes. One major challenge that hinders research into these differences is the inability to selectively isolate calcifying EVs from other vesicle populations. In this study, we hypothesized that the formation of mineral within calcifying EVs would increase the density of the vesicles such that they would pellet at a faster rate during ultracentrifugation. We show that after 10 min of ultracentrifugation at 100

SUBMITTER: Hutcheson JD 

PROVIDER: S-EPMC4261240 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature