A morphologic and semi-quantitative technique to analyze synthesis and release of specific proteins in cells.

ABSTRACT:

Background

With the rapid advancement of cell biology, the evaluation of a given protein's synthesis and release in cells becomes critical. However, up to now there has been no technique available to morphologically visualize and measure a newly synthesized protein in cells, nor can we measure the protein's release from the cells.

Results

In this study, we developed a set of assays combining pulse chase amino acid substitution, non-radioactive labeling, and immunofluorescence co-localization to visualize newly synthesized proteins in individual cells and then to detect their release using modified ELISA. We demonstrated the synthesis and release of Bcl-2, MMP-9, and immunoglobulin G (IgG) in a human trophoblast cell line, of which the last finding has not been reported previ

SUBMITTER: Huang G 

PROVIDER: S-EPMC4261763 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature