RAD9 deficiency enhances radiation induced bystander DNA damage and transcriptomal response.

ABSTRACT:

Background

Radiation induced bystander effects are an important component of the overall response of cells to irradiation and are associated with human health risks. The mechanism responsible includes intra-cellular and inter-cellular signaling by which the bystander response is propagated. However, details of the signaling mechanism are not well defined.

Methods

We measured the bystander response of Mrad9+/+ and Mrad9-/- mouse embryonic stem cells, as well as human H1299 cells with inherent or RNA interference-mediated reduced RAD9 levels after exposure to 1 Gy α particles, by scoring chromosomal aberrations and micronuclei formation, respectively. In addition, we used microarray gene expression analyses to profile the transcriptome of directly irradiated and bystander H129

SUBMITTER: Ghandhi SA 

PROVIDER: S-EPMC4261775 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature