Microalgal Metabolic Network Model Refinement through High-Throughput Functional Metabolic Profiling.

ABSTRACT: Metabolic modeling provides the means to define metabolic processes at a systems level; however, genome-scale metabolic models often remain incomplete in their description of metabolic networks and may include reactions that are experimentally unverified. This shortcoming is exacerbated in reconstructed models of newly isolated algal species, as there may be little to no biochemical evidence available for the metabolism of such isolates. The phenotype microarray (PM) technology (Biolog, Hayward, CA, USA) provides an efficient, high-throughput method to functionally define cellular metabolic activities in response to a large array of entry metabolites. The platform can experimentally verify many of the unverified reactions in a network model as well as identify missing or new reactions in t

SUBMITTER: Chaiboonchoe A 

PROVIDER: S-EPMC4261833 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature