MicroRNA expression profile in exosome discriminates extremely severe infections from mild infections for hand, foot and mouth disease.

ABSTRACT:

Background

Changes of miRNAs in exosome have been reported in different disease diagnosis and provided as potential biomarkers. In this study, we compared microRNA profile in exosomes in 5 MHFMD and 5 ESHFMD as well as in 5 healthy children.

Methods

Different expression of miRNAs in exosomes across all the three groups were screened using miRNA microarray method. Further validated test was conducted through quantitative real-time PCR assays with 54 exosome samples (18 ESHFMD, 18 MHFMD, and 18 healthy control). The judgment accuracy was then estimated by the receiver operating characteristic (ROC) curve analysis; and the specificity and sensitivity were evaluated by the multiple logistic regression analysis.

Results

There were 11 different miRNAs in exosomes of MHFMD a

SUBMITTER: Jia HL 

PROVIDER: S-EPMC4262082 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature