The mitochondrial genome of Parascaris univalens--implications for a "forgotten" parasite.

ABSTRACT:

Background

Parascaris univalens is an ascaridoid nematode of equids. Little is known about its epidemiology and population genetics in domestic and wild horse populations. PCR-based methods are suited to support studies in these areas, provided that reliable genetic markers are used. Recent studies have shown that mitochondrial (mt) genomic markers are applicable in such methods, but no such markers have been defined for P. univalens.

Methods

Mt genome regions were amplified from total genomic DNA isolated from P. univalens eggs by long-PCR and sequenced using Illumina technology. The mt genome was assembled and annotated using an established bioinformatic pipeline. Amino acid sequences inferred from all protein-encoding genes of the mt genomes were compared with those from

SUBMITTER: Jabbar A 

PROVIDER: S-EPMC4262126 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature