Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids).

ABSTRACT:

Background

Although spargana, which are the plerocercoids of Spirometra erinacei, are of biological and clinical importance, expressed sequence tags (ESTs) from this parasite have not been explored. To understand molecular and biological features of this parasite, sparganum ESTs were examined by large-scale EST sequencing and multiple bioinformatics tools.

Methods

Total RNA was isolated from spargana and then ESTs were generated, assembled and sequenced. Many biological aspects of spargana were investigated using multi-step bioinformatics tools.

Results

A total of 5,634 ESTs were collected from spargana. After clustering and assembly, the functions of 1,794 Sparganum Assembled ESTs (SpAEs) including 934 contigs and 860 singletons were analyzed. A total of 1,351 (75%)

SUBMITTER: Kim DW 

PROVIDER: S-EPMC4262225 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature