A fine-scale phylogenetic analysis of free-living Burkholderia species in sugarcane field soil.

ABSTRACT: The diversity and abundance of Burkholderia species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus Burkholderia. Nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. Quantitative PCR analysis revealed a consistent number of 16S rRNA gene copies for Burkholderia species (10(7) g(-1) soil) across the sampled fields. C/N, pH, and nitrate concentrations were important factors shaping the Burkholderia community structure; however, their impacts were not significant considering the overall genus size.

SUBMITTER: Tago K 

PROVIDER: S-EPMC4262370 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature