Solution hybrid selection capture for the recovery of functional full-length eukaryotic cDNAs from complex environmental samples.

ABSTRACT: Eukaryotic microbial communities play key functional roles in soil biology and potentially represent a rich source of natural products including biocatalysts. Culture-independent molecular methods are powerful tools to isolate functional genes from uncultured microorganisms. However, none of the methods used in environmental genomics allow for a rapid isolation of numerous functional genes from eukaryotic microbial communities. We developed an original adaptation of the solution hybrid selection (SHS) for an efficient recovery of functional complementary DNAs (cDNAs) synthesized from soil-extracted polyadenylated mRNAs. This protocol was tested on the Glycoside Hydrolase 11 gene family encoding endo-xylanases for which we designed 35 explorative 31-mers capture probes. SHS was implemented

SUBMITTER: Bragalini C 

PROVIDER: S-EPMC4263301 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature