Superresolution imaging reveals structural features of EB1 in microtubule plus-end tracking.

ABSTRACT: Visualization of specific molecules and their interactions in real time and space is essential to delineate how cellular dynamics and the signaling circuit are orchestrated. Spatial regulation of conformational dynamics and structural plasticity of protein interactions is required to rewire signaling circuitry in response to extracellular cues. We introduce a method for optically imaging intracellular protein interactions at nanometer spatial resolution in live cells, using photoactivatable complementary fluorescent (PACF) proteins. Subsets of complementary fluorescent protein molecules were activated, localized, and then bleached; this was followed by the assembly of superresolution images from aggregate position of sum interactive molecules. Using PACF, we obtained precise localization o

SUBMITTER: Xia P 

PROVIDER: S-EPMC4263457 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature