Comparative proteomic analysis of labellum and inner lateral petals in Cymbidium ensifolium flowers.

ABSTRACT: The labellum in orchids shares homology with the inner lateral petals of the flower. The labellum is a modified petal and often distinguished from other petals and sepals due to its large size and irregular shape. Herein, we combined two-dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization time of flight/time of flight (MALDI-TOF/TOF) approaches to identify the differentially expressed proteome between labellum and inner lateral petal in one of Orchid species (C. ensifolium). A total of 30 protein spots were identified, which showed more than a two-fold significant difference (p<0.05) in their expression. Compared with C. ensifolium transcriptome (sequenced in house), 21 proteins matched the translated nucleotide. The proteins identified were classified in

SUBMITTER: Li X 

PROVIDER: S-EPMC4264144 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature