A novel method for detection of HBVcccDNA in hepatocytes using rolling circle amplification combined with in situ PCR.

ABSTRACT:

Background

Intrahepatic hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) is the original template for HBV replication. The persistence of cccDNA is responsible for the recurrence of HBV infection. The detection of cccDNA can help the development of new antiviral drugs against HBV replication links, and reduce the resistance and recurrence as well as to discover extrahepatic HBV infection. In situ polymerase chain reaction (IS-PCR) can be used to determine the distribution and localization of cccDNA in liver tissues, but it is hampered by its low sensitivity and specificity. We developed a novel method to detect HBV cccDNA using rolling circle amplification (RCA) combined with IS-PCR.

Methods

Biopsy liver tissues were obtained from 26 patients with HBV infectio

SUBMITTER: Zhong Y 

PROVIDER: S-EPMC4264245 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature