Interrogation of gossypol therapy in glioblastoma implementing cell line and patient-derived tumour models.

ABSTRACT:

Background

Glioblastoma (GBM), being a highly vascularised and locally invasive tumour, is an attractive target for anti-angiogenic and anti-invasive therapies. The GBM/endothelial cell response to gossypol/temozolomide (TMZ) treatment was investigated with a particular aim to assess treatment effects on cancer hallmarks.

Methods

Cell viability, endothelial tube formation and GBM tumour cell invasion were variously assessed following combined treatment in vitro. The U87MG-luc2 subcutaneous xenograft model was used to investigate therapeutic response in vivo. Viable tumour response to treatment was interrogated using immunohistochemistry. Combined treatment protocols were also tested in primary GBM patient-derived cultures.

Results

An endothelial/GBM cell viability inh

SUBMITTER: Jarzabek MA 

PROVIDER: S-EPMC4264441 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature