Vimentin is a dominant target of in situ humoral immunity in human lupus tubulointerstitial nephritis.

ABSTRACT:

Objective

In lupus nephritis (LN), severe tubulointerstitial inflammation (TII) predicts progression to renal failure. Severe TII is associated with tertiary lymphoid neogenesis and in situ antigen-driven clonal B cell selection. The autoantigen(s) driving in situ B cell selection in TII are not known. This study was undertaken to identify the dominant driving autoantigen(s).

Methods

Single CD38+ or Ki-67+ B cells were laser captured from 7 biopsy specimens that were diagnostic for LN. Eighteen clonally expanded immunoglobulin heavy- and light-chain variable region pairs were cloned and expressed as monoclonal antibodies. Seven more antibodies were cloned from flow-sorted CD38+ cells from an eighth biopsy specimen. Antigen characterization was performed using a combination o

SUBMITTER: Kinloch AJ 

PROVIDER: S-EPMC4264660 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature